HEK293T cells were cultured in DMEM (Corning ... or HMGCR (Addgene, Cat# 86085) by using Lipofectamine 3000™ Transfection Reagent (ThermoFisher, Cat# L3000008). The cells were subsequently treated ...

(57,58) Finally, we examined the transfection efficiency of LNPs containing either ... However, the first and second heating scans were identical for mRNA/LNPs. HEK293T cells were cultured in RPMI1640 ...

(B) HEK293T cells were cotransfected with MmMYL3 ... were transfected with different plasmids using Lipofectamine 8000 (Beyotime) following the manufacturer’s instructions. At 24 h post-transfection, ...

HEK293T and HEK293TT cells were transiently transfected with plasmids ... while HeLa cells were transfected with Lipofectamine 2000 (Invitrogen, USA). Both transfection reagents were applied at ...

5 puro (Addgene) and confirmed via sequencing analysis. Lentivirus was produced by transient co‐transfection of HEK293T cells with a lentivirus vector, packaging plasmid, psPAX2, and pMD2.G using PEI ...

These movements are difficult for people with Parkinson’s disease. The disorder eats away at brain cells—called dopamine neurons—that control movement and emotion. Symptoms begin with tremors. Then ...

Fill out the form below and we'll send your colleague an invitation to the "Advanced Strategies for Effective mRNA Transfection in T Cells" event. Optimizing mRNA transfection in human T cells is a ...

Chinese solar module manufacturer Longi has revealed it achieved a power conversion efficiency of 34.85% for a two-terminal tandem perovskite solar cell. The U.S. Department of Energy's National ...

Hamas urged Jordan to release members of a reported Muslim Brotherhood terrorist cell, who were arrested last week by the Jordanian Intelligence, after being accused of attempting to manufacture ...