Close the pen drive's disk window when you finish, and then click the "Safely Remove Hardware" icon on the taskbar – it looks like a USB plug with a white check mark in a green circle next to it.

Method #3: Format the Virus Infected USB Drive to Remove Virus from pen drive Ultimately, this is the only way to resolve the common issue such as remove virus from pen drive without loosing data.

Most SD cards/pen drives still come formatted in FAT32. After coming across all the advantages, NTFS provides over the older format you might be wondering why.

USB Disk Storage Format Tool utility allows you to quickly and thoroughly format virtually any USB flash drive with your choice of FAT, FAT32, exFAT, or NTFS partition types.