This core performs non-radioactive, RNA in situ hybridization (ISH) on tissue sections. A unique high-throughput technology developed by the core (Yaylaoglu MB, Titmus A, Visel A, Alvarez-Bolado G, ...

All steps of ISH – from pre-hybridization to additions of probes, hybridization, stringency washes and antibody-mediated chromogenic or fluorescent reaction – are carried out by the robotic system.

Traditionally, HER2 status has been determined by immunohistochemistry (IHC) and in situ hybridization (ISH), distinguishing ...

Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that enables the detection and location of specific DNA sequences on chromosomes. 1 It is also used to detect the ...

(a) The basic elements of FISH are a DNA probe and a target sequence. (b) Before hybridization, the DNA probe is labeled by various means, such as nick translation, random primed labeling ...

When a species reproduces, typically, each parent passes on one of their two versions (alleles) of a given gene to their ...

Chromosomes occupy specific regions of a nucleus, called "chromosome territories." These subdomains might be doing much more than just keeping everything organized.

Researchers at Peking University have discovered that a filamentous gut fungus, Fusarium foetens, can reverse metabolic ...

A healthy 60-year-old man presented with a recurrent lesion on his left third finger after surgical treatment 7 years previously (Figure 1). A biopsy reveals squamous cell carcinoma (SCC) in situ.

Precision Cancer Pathology group performs RNA in situ hybridization for formalin fixed paraffin embedded tissue sections, fixed cultured cells and fixed/frozen tissue sections. We use either ...